| 摘要 |
The invention relates to a variant of a parent Termamyl-like ±-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca 2+ concentrations (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an ±-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an ±-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an ±-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an ±-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like ±-amylase, which variant exhibits increased thermostability at acidic pH and/or at low Ca 2+ concentrations (relative to the parent). |
