发明名称 |
CHARACTERIZATION OF INDIVIDUAL POLYMER MOLECULES BASED ON MONOMER-INTERFACE INTERACTIONS |
摘要 |
The invention relates to a method for characterizing a target polynucleic acid by providing a surface containing a channel of a dimension sufficient to allow sequential monomer-by-monomer passage of a single-stranded polynucleic acid, but not of a double-stranded polynucleic acid; providing a source of hybridized target polynucleic acid at the surface; inducing passage of the target polynucleic acid through the channel, whereby the target polynucleic acid undergoes base pair separation (melts) prior to its passage; and making one or more measurements over time as the target polynucleic acid moves relative to the channel yielding data suitable to determine a monomer-dependent characteristic of the target polynucleic acid. |
申请公布号 |
US2013313112(A1) |
申请公布日期 |
2013.11.28 |
申请号 |
US201313962141 |
申请日期 |
2013.08.08 |
申请人 |
PRESIDENT AND FELLOWS OF HARVARD COLLEGE |
发明人 |
DENISON TIMOTHY J.;SAUER-BUDGE ALEXIS;GOLOVCHENKO JENE A.;MELLER AMIT;BRANDIN ERIC;BRANTON DANIEL |
分类号 |
G01N27/447 |
主分类号 |
G01N27/447 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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