发明名称 |
METHOD OF IMMOBILIZING AND PROCESSING FUNCTIONAL MULTICOMPONENT STRUCTURES OF THE EXTRACELLULAR MATRIX |
摘要 |
The invention relates to a method of immobilizing and processing functional multicomponent structures of the extracellular matrix (ECM), comprising the following successive process steps: a) covalent binding of an adhesion promoter layer to cell culture carriers; b) culturing cells of a desired type on the adhesion promoter layer and thus immobilizing the extracellular matrix (ECM) secreted by the cells by secretion and binding to the adhesion promoter layer; and c) application of a decellurization protocol so as to detach matrix-secreting cells from the surface while simultaneously retaining the structure and functionality of the immobilized extracellular matrix (ECM) which is connected to the adhesion promoter and which lies below it. |
申请公布号 |
EP2665810(A1) |
申请公布日期 |
2013.11.27 |
申请号 |
EP20120700553 |
申请日期 |
2012.01.11 |
申请人 |
LEIBNIZ-INSTITUT FUER POLYMERFORSCHUNG DRESDEN E.V. |
发明人 |
PREWITZ, MARINA;FREUDENBERG, UWE;WERNER, CARSTEN |
分类号 |
C12N5/00 |
主分类号 |
C12N5/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|