发明名称 |
RECOMBINANT STRAIN Escherichia coli-PRODUCER OF HYDROLASE OF ALPHA-AMINO ACIDS ESTERS FROM Xanthomonas rubrilineans, METHOD FOR MICROBIOLOGICAL SYNTHESIS OF HYDROLASE OF ALPHA-AMINO ACIDS ESTERS BASED ON ABOVE SAID STRAIN |
摘要 |
FIELD: biotechnologies.SUBSTANCE: strain is built by transformation of strain-recipient Escherichia coli BL21 (DE3) with plasmid DNA containing the coding area of gene aehR of hydrolase of alpha-amino acids esters Xanthomonas rubrilineans All-Russian collection of industrial microorganisms B-9915 under control of promoter T7 and gene of stability to kanamycin Kan of hydrolase of alpha amino acids esters from Xanthomonas rubrilineans All-Russian collection of industrial microorganisms B-9915. The proposed synthesis method of hydrolase of alpha-amino acids esters is implemented by cultivation of strain-producer Escherichia coli All-Russian collection of industrial microorganisms B-11246 with addition to the composition of environment of kanamycin and isopropyl-"?"-D-tiogallactoside as an expression inducer of the obtained product.EFFECT: high biosynthesis level of target product, which can be used for development of methods for obtaining biocatalysts of synthesis processes of aminopenicillins and aminocephalosporins.2 cl, 3 dwg, 1 tbl, 5 ex |
申请公布号 |
RU2499830(C1) |
申请公布日期 |
2013.11.27 |
申请号 |
RU20120120576 |
申请日期 |
2012.05.18 |
申请人 |
FEDERAL'NOE GOSUDARSTVENNOE UNITARNOE PREDPRIJATIE "GOSUDARSTVENNYJ NAUCHNO-ISSLEDOVATEL'SKIJ INSTITUT GENETIKI I SELEKTSII PROMYSHLENNYKH MIKROORGANIZMOV" (FGUP "GOSNIIGENETIKA");OBSHCHESTVO S OGRANICHENNOJ OTVETSTVENNOST'JU "INNOVATSII I VYSOKIE TEKHNOLOGII MGU" (OOO "INNOTEKHMGU") |
发明人 |
BEREZINA OKSANA VALENTINOVNA;SKLJARENKO ANNA VLADIMIROVNA;ABESHEVA ZAJAN ANDREEVNA;SATAROVA DZHENNI EHRNSTOVNA;JAROTSKIJ SERGEJ VIKTOROVICH;TISHKOV VLADIMIR IVANOVICH;FEDORCHUK VLADIMIR IVANOVICH;FEDORCHUK ELENA ALEKSANDROVNA;SAVIN SVJATOSLAV SERGEEVICH |
分类号 |
C12N1/21;C12N9/14;C12P35/00 |
主分类号 |
C12N1/21 |
代理机构 |
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