| 摘要 |
FIELD: medicine.SUBSTANCE: invention relates to veterinary science. A method for preparing a bacterial preparation for preventing bovine postpartum endometritis consisting in the fact that preparing involves making a dry nutrient broth for microorganism culture (DNB) according to the package insert; the prepared broth is added with 3% starch pre-dissolved in a small amount of the cooled broth, and then mixed with the rest DNB; the broth is brought to the boiling point, boiled to produce a homogenous transparent mass; then the medium is added with 0.125% NaCl and 2% glucose to pH 6-7, then a phosphate buffer is added: one-substituted 0.8% potassium KHPOand 0.18% two-substituted potassium or sodium KHPOor NaHPO; the solution is boiled for 15 minutes, and after cooling, it is added with 0.5% of 80% lactic acid, filtered and autoclaved at 0.5 atm. for 30-40 min; after the solution has been cooled, it is added with the prepared cultures of Lactobacillus fermentum 44/1 and Lactococcus lactis subsp.Lactis 57no 1 bln. of the microbial cells per 100 ml of the medium in the sterile environment; it is packaged and thermostated for 48 hours at t=37°C. The preparation is tested in 56 cows for the purpose of preventing bovine postpartum endometritis.EFFECT: invention provides reducing the cost price of the preparation as compared to its prototype by 60%, without impairing the prophylactic efficacy, eliminates using antibiotics and derivatives thereof, sulphanyl amides, nitrofuranes and thereby eliminates side action of the preparation on animal's body.1 tbl, 2 ex |
