发明名称 |
Methods and Compositions for Dual Extraction of Protein and Nucleic Acid |
摘要 |
The present invention provides a method of isolating nucleic acid and protein from the same biological sample, comprising, in the following order: a) disrupting the biological sample; b) contacting the disrupted biological sample of (a) with a protein lysis buffer that lacks any component that denatures or reduces protein to produce a first lysate; c) centrifuging the first lysate of (b) to produce a first supernatant containing protein and a pellet containing nucleic acid; d) removing the first supernatant of (c), thereby isolating protein from the biological sample; e) contacting the pellet of (d) with nucleic acid lysis buffer to produce a second lysate; f) centrifuging the second lysate of (e) to produce a second supernatant containing nucleic acid; and g) removing the second supernatant of (f), thereby isolating nucleic acid from the same biological sample.
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申请公布号 |
US2013280718(A1) |
申请公布日期 |
2013.10.24 |
申请号 |
US201213450039 |
申请日期 |
2012.04.18 |
申请人 |
YARNALL MICHELE SUSAN;WHINNA RACHEL;WANG WENLING;HUANG JAMIE;SYNGENTA PARTICIPATIONS AG |
发明人 |
YARNALL MICHELE SUSAN;WHINNA RACHEL;WANG WENLING;HUANG JAMIE |
分类号 |
C07K1/14;C12Q1/68;G01N33/53 |
主分类号 |
C07K1/14 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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