| 摘要 |
PURPOSE: A method for isolating pilocarpine is provided to maximize the quantity of pilocarpine according to the combination of enzymes, temperature, and pH concentration. CONSTITUTION: A method for isolating pilocarpine comprises the steps of: pulverizing dried jaborandi leaves; mixing three times weight of distilled water with the pulverized jaborandi and preparing a mixture solution; adjusting the mixture solution to pH 3; heating the mixture solution at 100°C for one hour; adjusting the heated solution to pH 10.5; mixing the solution with twice weight of chloroform and inducing phase separation; isolating and concentrating a lower liquid from the solution; mixing the concentrated lower liquid with twice weight of sulfuric acid and inducing phase separation; isolating and adjusting a supernatant to pH 10.5; mixing the supernatant with twice weight of chloroform and inducing phase separation; isolating and concentrating a lower liquid; and adding a mixture of acetone and methanol to the concentrated lower liquid and extracting pilocarpine. [Reference numerals] (AA) Pulverizing jaborandi leaves; (BB) Mixing distilled water with the pulverized jaborandi leaves; (CC) Adjusting the mixture solution to pH 3; (DD) Heating the mixture solution at 100°C for one hour; (EE) Mixing the solution with chloroform and isolating a lower liquid from the solution; (FF) Concentrating the lower liquid; (GG) Concentrating the lower liquid/extracting pilocarpine; (HH) Adjusting a upper liquid to pH 10.5; (II) Isolating the upper liquid; (JJ) Mixing with sulfuric acid and separating phases |
