METHOD FOR PRODUCTION OF ANTIGEN FOR DIAGNOSTICS OF RESPIRATORY AVIAN MYCOPLASMOSIS BY ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA)

摘要

The method for production of antigen for diagnostics of respiratory avian mycoplasmosis by enzyme-linked immunosorbent assay (ELISA) comprises infection of passaged cell culture with the strain of Mycoplasma gallisepticum S, cultivation, isolation of cultural liquid, centrifugation. For accumulation of mycoplasma the trypsin fibroblast culture (CEF) is used, and threefold freezing-defrosting for antigen production.