| 摘要 |
FIELD: medicine, pharmaceutics.SUBSTANCE: invention refers to biotechnology and describes a method for preparing a purified concentrate of influenza virus; the method involves microfiltration of a virus-containing allantoic fluid in filter elements of the size of 0.1-0.2 mcm and further diafiltration in a phosphate buffer solution containing sodium chloride 0.25-0.5 M, EDTA or sodium citrate, concentration of influenza virus on membranes with a cuttoff threshold of 300-500 kDa and further diafiltration in a phosphate buffer solution containing sodium chloride 0.25-0.5 M, EDTA or sodium citrate, an anionic detergent 0.02-0.002%, and ultra-centrifugation in a density gradient of saccharose prepared in a phosphate buffer solution containing sodium chloride 0.25-0.5 M, EDTA or sodium citrate, and an anionic detergent 0.02-0.002%.EFFECT: preparing the high-purity concentrate of influenza virus having more than 80% yield, optimising the process.3 ex, 2 tbl |
