发明名称 |
Mutants of staphylokinase carrying amino and carboxy-terminal extensions for polyethylene glycol conjugation |
摘要 |
The present invention relates to the development of new derivatives of a bacterial plasminogen activator, Staphylokinase (SAK), having one or more amino acid residues with single or multiple cysteines at the amino and/or carboxy terminal ends and their conjugation with PEG (Polyethylene Glycol), resulting in new Staphylokinase derivatives that display altered oligomeric states, enhanced thermal and protease stability and extended plasma half-life. Also included is the cloning and expression in a suitable bacterial host; purification of Staphylokinase derivatives to homogeneity and their chemical modification by integrating a PEG molecule to create new biologically active Staphylokinases having higher protein stability and improved in vivo plasma half life, that may enhance the clinical potential of Staphylokinase in thrombolytic therapy for the treatment of cardiovascular diseases. |
申请公布号 |
EP2370574(B1) |
申请公布日期 |
2013.09.25 |
申请号 |
EP20090793576 |
申请日期 |
2009.12.04 |
申请人 |
COUNCIL OF SCIENTIFIC & INDUSTRIAL RESEARCH |
发明人 |
SINGH, SATISH;DIKSHIT, KANAK, LATA |
分类号 |
C12N9/48;A61K38/48;A61K47/48;C07K14/31;C12N1/21 |
主分类号 |
C12N9/48 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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