| 摘要 |
A chimeric gene, which comprises a gene encoding Ag85a protein of Mycobacterium tuberculosis and a gene encoding the 125-282 amino acids of Ag85b protein of Mycobacterium tuberculosis that inserts into the Kpn I and/or Acc I endonuclease recognition sequence of the Ag85a gene at 245-250 or 430-435 sites respectively, is provided. It provides Mycobacterium tuberculosis gene vaccine comprising said chimeric gene wherein said Ag85a gene is cloned into eukaryotic expression vector. It also provides a preparation method for said Mycobacterium tuberculosis gene vaccine, including: amplifying said Ag85b gene fragment by PCR, inserting said amplified fragment into the eukaryotic expression vector which comprises Ag85a gene, and then ligating them by ligase. The chimeric Mycobacterium tuberculosis gene vaccine of the present invention can be used for treating the infection of drug resistance Mycobacterium tuberculosis and inducing high intensity cell immune response to tuberculosis in combine with levamisole adjuvant. |
