发明名称 |
AMPLIFICATION METHODS TO MINIMISE SEQUENCE SPECIFIC BIAS |
摘要 |
Methods for amplifying nucleic acids are provided. The methods can be used to minimise sequence specific bias caused by the preferential amplification of certain nucleic acid sequences. Methods are described which can lower the efficiency of AT rich templates relative to GC rich templates, thereby minimising GC bias during amplification reactions with multiple templates of different sequence. The methods are suited to solid phase amplification, for example, utilising flow cells. |
申请公布号 |
EP2539464(A4) |
申请公布日期 |
2013.09.25 |
申请号 |
EP20110747961 |
申请日期 |
2011.02.23 |
申请人 |
ILLUMINA, INC. |
发明人 |
RIGATTI, ROBERTO;BOUTELL, JONATHAN MARK;SHEN, MIN-JUI RICHARD |
分类号 |
C12Q1/68;C12N15/10 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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