发明名称 |
C-TERMINAL MODIFICATION OF POLYPEPTIDES |
摘要 |
The invention relates to a mutated trypsin comprising an amino acid substitution both at position K60 and D189, and at least one more amino acid substitution by histidine at position N143 or position E151. Such trypsin mutant has a preferred cleavage site comprising the amino acids Xaal -Xaaz His, wherein Xaal is L, Y or F and Xaa2 is R or K. The invention also relates to a man-made polypeptide comprising a target peptide and the above cleavage site as well as to a method of producing C-terminally modified target peptides by using this mutated trypsin. |
申请公布号 |
CA2569707(C) |
申请公布日期 |
2013.09.24 |
申请号 |
CA20052569707 |
申请日期 |
2005.08.12 |
申请人 |
F. HOFFMANN-LA ROCHE AG |
发明人 |
HOESS, EVA;BORDUSA, FRANK;HERRMANN, RUPERT;JAKUBKE, HANS-DIETER;TISCHER, WILHELM |
分类号 |
C12N9/76;C12N15/10;C12Q1/37 |
主分类号 |
C12N9/76 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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