发明名称 |
METHOD FOR PRODUCING MINUS-STRAND RNA VIRAL VECTORS USING HYBRID PROMOTER COMPRISING CYTOMEGALOVIRUS ENHANCER AND CHICKEN .BETA.-ACTIN PROMOTER |
摘要 |
The present invention provides methods for producing a minus-strand RNA viral vector, which comprise using a promoter comprising a cytomegalovirus enhancer and a chicken .beta.-actin promoter, to induce the transcription of the genome RNA of a minus-strand RNA viral vector and the expression of minus-strand RNA viral proteins that form a ribonucleoprotein with the genome RNA. The methods of the present invention enable high efficiency production of highly safe minus-strand RNA viral vectors. The methods of the present invention are particularly useful for producing minus-strand RNA viral vectors that are deficient in envelope-constituting protein genes.
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申请公布号 |
CA2553976(C) |
申请公布日期 |
2013.09.24 |
申请号 |
CA20052553976 |
申请日期 |
2005.01.20 |
申请人 |
DNAVEC RESEARCH INC. |
发明人 |
IIDA, AKIHIRO;BAN, HIROSHI;INOUE, MAKOTO;HIRATA, TAKAHIRO;HASEGAWA, MAMORU |
分类号 |
C12N15/86;C12N5/10 |
主分类号 |
C12N15/86 |
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