发明名称 |
Phosphoramidites for synthetic RNA in the reverse direction, efficient RNA synthesis and convenient introduction of 3'-end ligands, chromophores and modifications of synthetic RNA |
摘要 |
The present invention provides building blocks and methods for synthesizing very pure RNA in a form that can efficiently be modified at the 3' end. Reverse RNA monomer phosphoramidites have been developed for RNA synthesis in 5'->3' direction, leading to very clean oligo synthesis that allows for the introduction of various modifications at the 3'-end cleanly and efficiently. Higher coupling efficiency per step have been observed during automated oligo synthesis with the reverse RNA amidites disclosed herein, resulting in a greater ability to achieve higher purity and produce very long oligonucleotides. The use of the reverse RNA phosphoramidites in the synthetic process of this invention leads to oligonucleotides free of N+1 species.
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申请公布号 |
US8541569(B2) |
申请公布日期 |
2013.09.24 |
申请号 |
US20100708827 |
申请日期 |
2010.02.19 |
申请人 |
SRIVASTAVA SURESH C.;SRIVASTAVA NAVEEN P.;CHEMGENES CORPORATION |
发明人 |
SRIVASTAVA SURESH C.;SRIVASTAVA NAVEEN P. |
分类号 |
C07H21/00 |
主分类号 |
C07H21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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