METHOD FOR PRODUCING BIOETHANOL FROM BANANA PSEUDOSTEM BY ENZYMATIC HYDROLYSIS, AND USE OF THE SAME

摘要

The present invention relates to a method for producing bioethanol from banana pseudostem by enzymatic hydrolysis, using a pre-treatment with sodium hydroxide pure or in combination with sodium hypochlorite, acetic acid and/or vinegar in combination with hydrogen peroxide, sulphuric acid, the combination of sodium hydroxide and sulphuric acid, the combination of vapour explosion and sodium hydroxide, followed by enzymatic hydrolysis of the biomass pre-treated with the NS 22074 and NS 50012 cellulase enzymes in an ultra-sound bath or shaker-type bath, the best result being obtained by enzymatic hydrolysis of the biomass in a shaker for 48 hours, with 3% sodium hydroxide (0.2 g), a mixture of the NS 22074 enzyme (0.015 mL) and NS 50012 enzyme (0.015 mL), since only 1.2 kg of pre-treated biomass and only 180 mL of the mixture of the NS 22074 and NS 50012 enzymes are required to produce 1 kg of fermentable sugars, thus proving to be an economically viable method for producing bioethanol.