Method for the production of cellulases based on the regulation of the oscillation of the pression of the oxygen disolved in the culture medium

摘要

Producing cellulolytic and/or hemicellulolytic enzyme by a cellulolytic microorganism in an aerated and agitated bioreactor containing growth phase in the presence of a carbon source and a production phase in the presence of an carbon inducing substrate in which the contribution of carbon inducing substrate during the production phase is regulated by oscillation of the partial pressure of oxygen dissolved in the middle of growth value of Po-2min to Po-2max, is claimed. Producing cellulolytic and/or hemicellulolytic enzyme by a cellulolytic microorganism in an aerated and agitated bioreactor containing growth phase in the presence of a carbon source and a production phase in the presence of an carbon inducing substrate in which the contribution of carbon inducing substrate during the production phase is regulated by oscillation of the partial pressure of oxygen dissolved in the middle of growth value of Po-2min to Po-2max, where the value of the Po2min is greater than the critical pressure (Po2crtic) below which the metabolism of the organism is affected and less than 95% of the partial pressure of oxygen saturation (Po2-sat) set at the start of the bioreactor, and Po2-max value is greater than at least 5% to Po2-min, is claimed.