| 发明名称 |
METHOD OF PRODUCTION OF INDUCED PLURIPOTENT STEM CELLS OF PATIENTS WITH DOWN SYNDROME |
| 摘要 |
FIELD: biotechnology.SUBSTANCE: method of production of induced pluripotent stem cells (IPS cells) from skin fibroblasts of patients with Down syndrome is proposed, which includes preliminary induction using lentiviral transfection with gene hTERT, further transfection of the resulting cells with three lentiviral constructs with genes Oct4, Sox2, Nanog, with subsequent cultivation for 14-21 days with valproic acid under conditions of low oxygen content 5% O.EFFECT: improvement of the method.2 ex |
| 申请公布号 |
RU2492233(C1) |
申请公布日期 |
2013.09.10 |
| 申请号 |
RU20120142244 |
申请日期 |
2012.10.04 |
| 申请人 |
ROSSIJSKAJA FEDERATSIJA, OT IMENI KOTOROJ VYSTUPAET MINISTERSTVO OBRAZOVANIJA I NAUKI ROSSIJSKOJ FEDERATSII;FEDERAL'NOE GOSUDARSTVENNOE BJUDZHETNOE UCHREZHDENIE NAUKI INSTITUT BIOLOGII RAZVITIJA IM. N.K. KOL'TSOVA ROSSIJSKOJ AKADEMII NAUK (IBR RAN) |
发明人 |
DASHINIMAEV EHRDEHM BAIROVICH;MUCHKAEVA IRINA ALEKSEEVNA;VASIL'EV ANDREJ VALENTINOVICH;TERSKIKH VASILIJ VASIL'EVICH;VISHNJAKOVA KHAVA SAFIULLOVNA |
| 分类号 |
C12N5/074 |
主分类号 |
C12N5/074 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
|
| 地址 |
|
