发明名称 |
HETERODIMERIC FUSION PROTEINS USEFUL FOR TARGETED IMMUNE THERAPY AND GENERAL IMMUNE STIMULATION |
摘要 |
Disclosed are methods for producing fusion proteins with the heterodimeric cytokine, interleukin-12. In order to insure that the proper ration of fused and non-fused subunits are obtained in the fusion protein, a specific stepwise approach to genetic engineering is used. This consists of first expressing the non-fused p40 IL-12 subunit in a production cell line, followed by or simultaneously expressing in the same cell, a second recombinant fusion protein consisting of the fused polypeptide linked by a peptide bond to the p35 subunit of IL-12. Molecules containing the p35 fusion protein cannot be secreted from the transfected mammalian cell without first complexing in a one to ratio with the p40 subunit, thus ensuring the production of active heterodimeric fusion proteins.
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申请公布号 |
CA2693296(C) |
申请公布日期 |
2013.09.10 |
申请号 |
CA19982693296 |
申请日期 |
1998.12.08 |
申请人 |
MERCK PATENT GMBH |
发明人 |
GILLIES, STEPHEN D.;LO, KIN-MING;LAN, YAN |
分类号 |
C07K19/00;C12N15/09;A61K38/00;A61K38/20;A61K39/00;A61K47/48;A61P37/04;C07K14/54;C07K16/28;C07K16/30;C12P21/00 |
主分类号 |
C07K19/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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