摘要 |
PROBLEM TO BE SOLVED: To improve a sterilizing process using gaseous biocide under vacuum.SOLUTION: A method of metering and supplying a preselected volume of hydrogen peroxide into a vessel under vacuum is disclosed. The method includes steps of: connecting a passage of known volume to the vessel under vacuum to evacuate the passage of known volume; sealing the passage; connecting the passage to a supply source of a hydrogen peroxide solution for a time sufficient to draw the hydrogen peroxide solution into the evacuated passage and fill the passage with the hydrogen peroxide solution; sealing the passage; and repeating the steps until a cumulative volume of fills of the passage is equal to the preselected volume. The known volume is preferably from 75 μL to 15 μL. Sterilization is controlled in an economic manner by obviating the need for a means to measure the hydrogen peroxide concentration. |