Antibody against partial protein comprising N-terminal region of PRDM 10 and method of preparing the same

摘要

PURPOSE: An antibody for n-terminus protein of PRDM 10 and a manufacturing method thereof are provided to be usefully used in the PRDM 10 protein function research. CONSTITUTION: An antibody for n-terminus protein of PRDM 10 is described in the sequence number 1(SEQ ID NO:1). The gene which codes the N- terminus protein has the base sequence described in the sequence number 2. The N- terminus protein of the PRDM 10 includes the zinc finger motif. A composition for detecting the PRDM 10 protein includes the antibody which unites with the N- terminus protein of the PRDM 10. The PRDM 10 related disease is inflammatory arthritis or cancer. A manufacturing method of the antibody for N terminus protein of the PRDM 10 comprises the following steps: processing PCR by using a primer in order to amplify the N terminous part of the PRDM 10 by using the PRDM 10 as a mold; ligating the amplified N terminous gene of the PRDM 10 and transforming into a host cell to over express the N-terminous protein of the RPDM 10; and obtaining the antibody for N-terminous protein of the RPDM 10 bt injecting the over expressed N terminus protein of the PRDM 10 into animals. [Reference numerals] (AA) Cloning expression using His tagging system; (BB) Antigen protein purification (IMAC); (CC) Immunization reaction induction inside rabbit body