IMMUNOASSAY FOR THE DETECTION OF C-TERMINAL FRAGMENTS OF AGRIN CONTAINING THE 22KDA C-TERMINAL FRAGMENT (CAF) OF AGRIN

摘要

Immunoassay for the detection of CAF (22-Kd fragment of agrin) in C-terminal fragments of agrin in a sample, in which the CAF in the fragments to be detected can be present in one or both of the following 2 different conformations CAF-closed and CAF-open, wherein - in a first step the sample is incubated with a (primary) antibody with known binding properties to the different conformations of the CAF, with the antibody being capable to specifically bind to at least the CAF-open conformation, and - in a second step it is determined whether or not antibody-CAF-complexes have been formed after incubation.