发明名称 |
HUMAN SWEET TASTE RECEPTOR AGONIST SWEETNESS REGULATING SUBSTANCE FOR SWEET SUBSTANCES |
摘要 |
[Object] An object of the present invention is to provide a human sweet taste receptor-acting sweet taste regulating substance to a sweet taste substance by which the advantageous effects such as improvement in a taste, saving of a sweetener, reduction in calorie, low caries etc. can be obtained by applying to various foods and beverages. [Solution] The sweet taste receptor-acting sweet taste regulating substance of the present invention is identified by measurement of a physiological response by a sweet taste substance, using a cultured cell strain which is allowed to express hT1R2 and hT1R3, and a G protein ± subunit, by transferring an expression construct obtained by inserting respective cDNAs encoding the hT1 R2 and the hT1 R3, and the G protein ± subunit into the same plasmid, into a 293 cell in which an FRT (Flippase Recognition Target) site has been incorporated into one place in a genome DNA. |
申请公布号 |
EP2508534(A4) |
申请公布日期 |
2013.08.28 |
申请号 |
EP20100834424 |
申请日期 |
2010.09.08 |
申请人 |
T. HASEGAWA CO., LTD.;THE UNIVERSITY OF TOKYO |
发明人 |
ABE, KEIKO;MISAKA, TAKUMI;FUJIWARA, SATOSHI |
分类号 |
G01N33/50;A23L27/00;A23L27/30;C07K14/705;C12N15/12;G01N33/566 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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