摘要 |
The present invention relates to a novel RNA-binding protein, to a composition comprising same for detecting microRNA, and to a label-free detection method for detecting microRNA using same. According to the present invention, the method for detecting microRNA using the RNA-binding protein, which is a fusion protein comprising a PAZ domain and a double stranded RNA-binding domain and which binds to double stranded RNA having an overhanging 3' terminal, is a label-free detection method for detecting microRNA without an enzymatic labeling process or enzymatic amplifying reaction, which may overcome the drawbacks of conventional microRNA analysis methods, that is, methods for analyzing microRNA after enzymatic labeling or methods for coupling an antibody to microRNA, wherein the drawbacks may include complicated processes, or difficulties developing an RNA-structure-specific antibody resulting from an instable RNA structure. Thus, the method of the present invention may be utilized in the study of gene expression in a variety of diseases, in the detection of diagnostic markers of diseases, and in the evaluation of drug efficacy. |