发明名称 |
REGULATED GENETIC SUICIDE MECHANISM COMPOSITIONS AND METHODS |
摘要 |
Embodiments of the present invention relates to the incorporation and use of a regulated genetic suicide mechanism for use in the improved purification of biologics, including adjunct use in various eubacterial minicell production and purification methodologies. Described herein are high-yield eubacterial minicell-producing strains with genetic modifications that comprise a regulated genetic suicide mechanism that irreparably destroys the parent cell chromosome such that live parental cells in a culture can be functionally eliminated at any time during the course of a minicell production and purification run. Embodiments of the present invention also describe methods useful in the elimination of live parental cells during the production of other cell-based biologics.
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申请公布号 |
US2013210121(A1) |
申请公布日期 |
2013.08.15 |
申请号 |
US201213711482 |
申请日期 |
2012.12.11 |
申请人 |
VAXIION THERAPEUTICS, INC.;VAXIION THERAPEUTICS, INC. |
发明人 |
GIACALONE MATTHEW J.;MALOY STANLEY;TSUJI SHINGO |
分类号 |
C12N15/74;C12N15/70 |
主分类号 |
C12N15/74 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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