发明名称 |
NOVEL ULTRASENSITIVE CELL BASED SENSORS AND USES THEREOF |
摘要 |
The present invention relates to a novel cell based sensor useful for drug discovery that comprises a cell line with professional regulated exocytosis of secretory granules transfected with a protease as a reporter polypeptide stored in the regulated secretory granules of the cell line with professional regulated exocytosis and having either an endogenous or a heterologous molecule as a modulator of regulated secretory granules exocytosis, such said granule stored protease reporter having at least: a high resistance to conditions already present inside the granules such as low pH and proteolysis by other proteases; enzymatic activity after exocytosis; a highly specific cleavage sequence; a very low level of secretion under unstimulated or basal conditions; and a high signal to background activity in a media compatible with cell culture viability and granule exocytosis for a high throughput robust and sensitive detection.
|
申请公布号 |
US2013203078(A1) |
申请公布日期 |
2013.08.08 |
申请号 |
US201013811872 |
申请日期 |
2010.07.28 |
申请人 |
PAZ-ROJAS ELIER;MONTERO-PENALVO MARIA DE GRACIA;LUNA-GUERRERO VERONICA INMACULADA;GARCIA MACEIRA FE ISABEL;MORALES-MARTINEZ JOSE ANDRES;GARCIA MACEIRA TANIA;ARAGON-GOMEZ ANA BELEN;QUESADA-MOLINA ANA;CANVAX BIOTECH SL |
发明人 |
PAZ-ROJAS ELIER;MONTERO-PENALVO MARIA DE GRACIA;LUNA-GUERRERO VERONICA INMACULADA;GARCIA MACEIRA FE ISABEL;MORALES-MARTINEZ JOSE ANDRES;GARCIA MACEIRA TANIA;ARAGON-GOMEZ ANA BELEN;QUESADA-MOLINA ANA |
分类号 |
G01N33/566;C12N15/85;C12Q1/37 |
主分类号 |
G01N33/566 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|