The transformation method of Liriodendron tulipifera embryonic tissue

摘要

PURPOSE: A method for transforming an embryogenic tissue of Liriodendron tulipifera is provided to study new variety of Liriodendron tulipifera. CONSTITUTION: A method for transforming an embryogenic tissue of Liriodendron tulipifera comprises: a step of co-culturing the embryogenic tissue with Agrobacterium; a step of treating the co-cultured embryogenic tissue with geneticin and cefotaxime and selecting transformed an embryogenic tissue; a step of treating the transformed embryogenic tissue with geneticin and cefotaxime and inducing somatic embryo; and a step of treating the somatic embryo with geneticin and cefotaxime.