CELL LINE OF LYMPHOCYTES COMPRISING GAMMA-DELTA T CELLS, COMPOSITION AND PRODUCTION METHOD THEREOF

摘要

The present invention relates to a cell line of lymphocytes comprising gammadelta T cells, composition and production method thereof, and medical use namely for the use in medicine, namely in cancer immunotherapy. The cell line comprise a sample of human peripheral blood Vdelta1+ gammadelta T cells expressing functional natural cytotoxicity receptors (NCRs). These Vdelta1+ NCR+ T lymphocytes can directly mediate killing of leukemia cell lines and chronic lymphocytic leukemia patient neoplastic cells. The present invention shows that human Vdelta1+ NCR+ T cells can be differentiated and expanded from total gammadelta peripheral blood lymphocytes (PBLs), upon regular in vitro or ex vivo stimulation with gammadeltaTauCR agonists and gammac-family cytokines. This subset surprisingly expresses NKp30, NKp44 and NKp46, and high levels of Granzyme B that associate with highly enhanced cytotoxicity against lymphoid leukemias.