METHOD TO DETERMINE ANTIBODIES TO OPPORTUNISTIC INFECTIONS IN LIQUOR OF CHILDREN

摘要

FIELD: biotechnologies.SUBSTANCE: diagnostic set is used to determine antibodies in blood, as well as polystyrene dishes. The solid liquor in the amount of 10 mcl is dissolved at the ratio of 1:100. The sample is incubated at 37° for 40-45 min. Secondary incubation with antibody conjugate is carried out at 37°-38° for 55-60 min. By variation of critical value of optical density they determine availability of antibodies in the liquor.EFFECT: method provides for determination of antibodies in children in a small sampled volume of liquor and may find application in diagnostics of opportunistic infections in children.4 tbl, 3 ex