| 摘要 |
In the present invention, a method for the qualitative genetic characterization and/or gene expression characterization of predetermined cells in a fluid sample containing such cells is provided. The inventive method for the qualitative genetic and/or gene expression characterization of predetermined cells in a fluid sample containing such cells, comprises: a) selectively extracting at least a part of the predetermined cells from the sample forming a cell suspension cso; and b) repeating the extraction step a) n times with the same sample of step a), with n >= 1, forming at least one cell suspension csn; c) determining a gene expression profile gepn and/or a first copy number count cnco of at least one DNA and/or RNA with at least a part of the cell suspension cso; d) determining at least one further gene expression profile gepn and/or a further copy number count cncn of at least one DNA and/or RNA with at least a part of at least one further cell suspension csn; e) calculating the predetermined cells' gene expression profile gep(P) of at least one predetermined DNA and/or RNA by subtracting gepn from gepo and/or the predetermined cells' copy number count cnc(P) of at least one predetermined DNA and/or RNA by subtracting cncn from cnco; and f) evaluating the qualitative genetic and/or gene expression characteristics of the predetermined cells from gep(P) and/or cnc(P). |
