发明名称 |
CELL CULTURE LIQUID/GAS-PHASE CHAMBER DEVICES AND RELATED METHODS, AND EXEMPLARY USE FOR MEASURING ACCUMULATION OF GAS-PHASE NITRIC OXIDE IN DIFFERENTIATED CULTURES OF NORMAL AND CYSTIC FIBROSIS AIRWAY EPITHELIAL CELLS |
摘要 |
<p>Embodiments disclosed herein include cell culture liquid/gas-phase chamber devices and related methods that allow measurement of gas-phase components and their production rate as well as substances/mediators released into media by cultured cells in response to various stimuli. In embodiments disclosed herein, these chamber devices are cell culture liquid/gas-phase chamber devices. The gas and liquid phase compartments are separated by the cell culture liquid/gas-phase chamber devices so that there is no, or minimal, physico-chemical interaction between the liquid and gas phase compartments. As an example, these cell culture liquid/gas-phase chamber devices can be used to study the effects of various pharmacologic agents or other interventions on healthy or diseased cell metabolism by assessing a release of metabolic products in liquid (culture media) and the headspace under standardized conditions. The reusable cell culture chamber devices allow for studying cultured cell responses under a short or prolonged incubation time.</p> |
申请公布号 |
WO2013106403(A1) |
申请公布日期 |
2013.07.18 |
申请号 |
WO2013US20789 |
申请日期 |
2013.01.09 |
申请人 |
UNIVERSITY OF NORTH CAROLINA |
发明人 |
HAZUCHA, MILAN, JOSEPH;CARSON, JOHNNY, LEE;OSTROWSKI, LAWRENCE, EDWARD |
分类号 |
C12M1/16;C12M1/04;C12N5/071 |
主分类号 |
C12M1/16 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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