Methods for converting prethrombin-1 to thrombin are disclosed. An aqueous solution of prethrombin-1 is applied to oscutarin-C immobilized on a solid support so as to provide from 500 mg to 4000 mg of prethrombin-1 per mL of the solid support and a contact time between the prethrombin-1 and the oscutarin-C of from 1.8 to 3.5 minutes. The resulting active thrombin may be captured on an ion exchange chromatography medium or an affinity chromatography medium.
申请公布号
CA2631715(C)
申请公布日期
2013.07.16
申请号
CA20062631715
申请日期
2006.12.21
申请人
ZYMOGENETICS, INC.
发明人
MALLET, ROBERT W.;STENLAND, CHRISTOPHER J.;BOONE, JONATHON C.;FORSTROM, JOHN W.;DE JONGH, KAREN S.