发明名称 |
Method of oligonucleotide sequencing by mass spectrometry |
摘要 |
A computer-implemented method for confirming the nucleotide sequence of an oligonucleotide is provided. In certain embodiments, the method comprises: a) inputting the nucleotide sequence of an oligonucleotide; b) executing an algorithm that provides the predicted molecular formulas of fragments of the oligonucleotide; c) comparing the predicted m/z values of the predicted molecular formulas to experimentally-obtained m/z values obtained by analysis of the oligonucleotide by tandem mass spectrometry to determine if the predicted masses correspond with the experimentally-obtained masses. The method may be used, for example, to confirm the identity of an oligonucleotide after it is synthesized.
|
申请公布号 |
US8486707(B2) |
申请公布日期 |
2013.07.16 |
申请号 |
US201113093723 |
申请日期 |
2011.04.25 |
申请人 |
TIMAR ZOLTAN;HEGEDUS GERGO;AGILENT TECHNOLOGIES, INC. |
发明人 |
TIMAR ZOLTAN;HEGEDUS GERGO |
分类号 |
H01J49/26;G01N21/00;G06F19/00 |
主分类号 |
H01J49/26 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|