Competitive biosensor having elevated sensitivity

摘要

The invention relates to measures for determining glucose and for diagnosing diseases that are based on impaired glucose metabolism. In particular, the invention relates to a device comprising a hydrogel, having a glucose-binding protein incorporated therein and a ligand of the glucose-binding protein, wherein the hydrogel comprises a first hydrogel matrix made of alginate and a second hydrogel matrix, which forms an interpenetrating network within the first hydrogel matrix. The invention further relates to the use of such a device for determining the glucose content in a sample and to the use of the device for diagnosing impaired glucose metabolism in a test subject.