Method of constructing masses of myocardial cells and use of the myocardial cell mass

摘要

It is intended to improve the post-transplantation taking rate of myocardial cells which have been purified so that they are composed exclusively of myocardial cells and free from cells originating in other species. To solve the above problem, it has been discussed whether or not a cell mass of purified myocardial cells can be constructed. As the results, it has been clarified that the above-described problem can be solved by providing a method for constructing a cell of pluripotent stem cell-origin myocardial cells characterized by comprising dissociating an aggregated cell mass containing myocardial cells, which have been differentiated and derived from pluripotent stem cells, to give individual single cells, incubating the pluripotent stem cell-origin myocardial cells having been thus purified by using a medium under serum-free conditions and thus aggregating the cells.