FERMENTATIVE PRODUCTION OF FINE CHEMICALS

摘要

THE INVENTION RELATES TO A PROCESS FOR THE PRODUCTION OF AT LEAST ONE MICROBIAL METABOLITE HAVING AT LEAST 3 CARBON ATOMS OR AT LEAST 2 CARBON ATOMS AND AT LEAST 1 NITROGEN ATOM BY MEANS OF SUGAR-BASED MICROBIAL FERMENTATION, COMPRISING: D) THE PREPARATION OF A SUGAR-CONTAINING LIQUID MEDIUM WITH A MONOSACCHARIDE CONTENT OF MORE THAN 20% BY WEIGHT FROM A STARCH FEEDSTOCK, THE SUGAR-CONTAINING LIQUID MEDIUM ALSO COMPRISING NON-STARCHY SOLID CONSTITUENTS OF THE STARCH FEEDSTOCK; E) THE FERMENTATION OF THE SUGAR-CONTAINING LIQUID MEDIUM FOR THE PRODUCTION OF THE METABOLITE(S); AND F)DEPLETION OR ISOLATION OF AT LEAST ONE METABOLITE FROM THE FERMENTATION LIQUOR, WHEREIN A MICROORGANISM STRAIN WHICH PRODUCES THE DESIRED METABOLITE(S) IS CULTIVATED WITH THE SUGAR-CONTAINING LIQUID MEDIUM, SAID LIQUID MEDIUM BEING OBTAINED BY: A3) MILLING THE STARCH FEEDSTOCK; AND A4) LIQUEFYING THE MILLBASE IN AN AQUEOUS LIQUID IN THE PRESENCE OF AT LEAST ONE STARCH-LIQUEFYING ENZYME, FOLLOWED BY SACCHARIFICATION USING AT LEAST ONE SACCHARIFYING ENZYME, WHERE AT LEAST SOME OF THE MILLBASE IS LIQUEFIED BY CONTINUOUS OR BATCHWISE ADDITION TO THE AQUEOUS LIQUID.