The invention relates to a method for providing analogs of human milk oligosaccharides (HMO), in particular oligosaccharides containing terminal sialic acid. The method comprises the steps of : a) providing a source of non-digestible galactooligosaccharides (GOS) containing at least two terminally bonded b-linked galactose residues; b) providing a sialic acid donor having (a2-3)-sialylated O-glycans; c) contacting said GOS with said sialic acid donor in the presence of an enzyme having trans-sialidase activity in an enzyme reaction mixture; and d) isolating from said enzyme reaction mixture a fraction comprising at least 20 percent by weight of disialylated galactooligosaccharides (di-Sia-GOS) based on the dry matter.
申请公布号
CA2871294(A1)
申请公布日期
2013.06.13
申请号
CA20122871294
申请日期
2012.12.06
申请人
FRIESLAND BRANDS B.V.;RIJKSUNIVERSITEIT GRONINGEN;DARLING INGREDIENTS NEDERLAND B.V.