Methods of increasing dihydroxy acid dehydratase activity to improve production of fuels, chemicals, and amino acids

摘要

A recombinant Saccharomyces cerevisiae comprising a recombinantly overexpressed polynucleotide encoding a cytosol-localised dihydroxy acid dehydratase (DHAD) and at least one modification which increases the activity of S. cerevisiae Aft1 (activator of ferrous transport) protein which increeases the dehydratase activity of DHAD. The modification may be a mutation of Cys 291 Phe 291.