Escherichia coli expressing the cytochrome P-450 gene and a method for microbial conversion using them

摘要

Disclosed is a means for improving the poor conversion efficiency in a bioconversion system using an Escherichia coli cell having a bacterium-originated cytochrome P-450 gene integrated therein. A recombinant Escherichia coli cell is produced by introducing aciB and aciC which encode a gene for the electron transport system originated from the Acinetobacter sp. OC4 strain into an Escherichia coli cell, and adding a polynucleotide encoding an N-terminal sequence composed of 48 amino acid residues of AciA and the like to the 5'-terminus of a bacterium-originated cytochrome P-450 gene, wherein AciA is an alkane-oxidative cytochrome P-450 originated from the Acinetobacter sp. OC4 strain. Use of the recombinant Escherichia coli cell results in much effective microbial conversion of a hydrophobic or amphipathic substrate compound into a desired compound.