A YEAST ARTIFICIAL CHROMOSOME CARRYING THE MAMMALIAN GLYCOSYLATION PATHWAY

摘要

A Yeast Artificial Chromosome (YAC) directing the expression of one or more activities of the humanized glycosylation pathway is provided. The said YAC comprises one or more expression cassettes for fusion proteins of heterologous glycosylation pathway and an ER/Golgi retention sequence. The invention also relates to new yeast cells which contain the said YAC. Finally, the invention also provides a method for producing recombinant target glycoproteins.