发明名称 |
VASCULAR PROGENITOR CELL SHEET DERIVED FROM INDUCED PLURIPOTENT STEM CELLS, AND PRODUCTION METHOD THEREFOR |
摘要 |
The present invention addresses the problem of providing a vascular progenitor cell sheet derived from induced pluripotent stem cells, which has the strength to tolerate practical applications and exhibits a high treatment effect. This vascular progenitor cell sheet derived from induced pluripotent stem cells is prepared by performing: (1) a step for preparing magnetically labeled Flk-1 positive cells derived from induced pluripotent stem cells; (2) a step for preparing a mixture of the Flk-1 positive cells and a gel material including type I collagen, laminin, type IV collagen and entactin as active ingredients, and then disseminating the mixture in a culture vessel; (3) a step for drawing the Flk-1 positive cells in the mixture to the culture surface of the culture vessel by application of a magnetic force to form a multi-layered cell layer; and (4) a step for gelling the gel material. |
申请公布号 |
WO2013069661(A1) |
申请公布日期 |
2013.05.16 |
申请号 |
WO2012JP78787 |
申请日期 |
2012.11.07 |
申请人 |
NATIONAL UNIVERSITY CORPORATION NAGOYA UNIVERSITY |
发明人 |
MUROHARA TOYOAKI;HONDA HIROYUKI;SHIBATA REI;ISHII MASAKAZU;KITO TETSUTARO;SUZUKI HIROHIKO |
分类号 |
C12N5/10;A61K35/12;A61L27/00;A61P9/00;A61P9/10;A61P17/02;C12M1/00;C12M3/04 |
主分类号 |
C12N5/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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