发明名称 |
Methods and compositions for DNA manipulation |
摘要 |
single-stranded extension having a desired length and sequence composition. Methods for forming single-stranded extensions include: the use of a cassette containing at least one nicking site and at least one restriction site at a predetermined distance from each other and in a predetermined orientation; or primer-dependent amplification which introduces into a polynucleotide molecule, a modified nucleotide which is excised to create a nick using a nicking agent. The methods and compositions provided can be used to manipulate a DNA sequence including introducing site specific mutations into a polynucleotide molecule and for cloning any polynucleotide molecule or set of joined polynucleotide molecules in a recipient molecule such as a vector of choice. |
申请公布号 |
US8440401(B2) |
申请公布日期 |
2013.05.14 |
申请号 |
US201113052536 |
申请日期 |
2011.03.21 |
申请人 |
BITINAITE JURATE;NEW ENGLAND BIOLABS, INC. |
发明人 |
BITINAITE JURATE |
分类号 |
C12Q1/68;C07H21/02;C07H21/04;C12N9/22;C12N15/10;C12N15/64;C12N15/66 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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