| 摘要 |
Provided is a fluorescence observation apparatus including an illumination unit that irradiates an object with illumination light and excitation light; a fluorescence-image acquisition unit that captures an image of the fluorescence generated by the object, thereby acquiring a fluorescence image; a return-light-image acquisition unit that captures an image of the return light returning from the object, thereby acquiring a return-light image; a light-distribution-characteristics-information storage unit that stores information with regard to the light distribution characteristics of optical systems; an image correcting unit that corrects at least one of the fluorescence image and the return-light image using the information so that the light distribution characteristics contained in the fluorescence image and the return-light image are made equal to each other; and an image normalizing unit that normalizes the fluorescence image on the basis of the return-light image, using the corrected fluorescence image and return-light image.
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