发明名称 |
Digital Counting of Individual Molecules by Stochastic Attachment of Diverse Label-Tags |
摘要 |
Compositions, methods and kits are disclosed for high-sensitivity counting of individual molecules by stochastic labeling of a identical molecules in mixtures of molecules by attachment of a unique label-tags from a diverse pool of label tags to confer uniqueness to otherwise identical or indistinguishable events. Individual occurrences of target molecules randomly choose from a non-depleting reservoir of diverse label-tags. Labeled molecules may be detected by hybridization or sequencing based methods. Molecules that would otherwise be identical in information content are labeled to create a separately detectable product that can be distinctly detected. The disclosed stochastic transformation methods reduce the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed label-tags are present. The methods may be used, for example, to count a given species of molecule within a sample.
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申请公布号 |
US2013116130(A1) |
申请公布日期 |
2013.05.09 |
申请号 |
US201113327526 |
申请日期 |
2011.12.15 |
申请人 |
FU GLENN K.;FODOR STEPHEN P.A.;AFFYMETRIX, INC. |
发明人 |
FU GLENN K.;FODOR STEPHEN P.A. |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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