摘要 |
<p>Method of determining the potentially available activity of cathepsin B in a biological sample, including the activity of the active form of cathepsin B, the activable form of cathepsin B from the proform procathepsin B which is present in the sample and the activable form of cathepsin B which is present in the sample in a form in which it is inhibited in respect of its protease activity by protease inhibitor, where procathepsin B present in the sample is converted into the active form cathepsin B, the content of free cathepsin-B-protease inhibitor present in the sample is reduced, or the inhibitor function of free cathepsin-B-protease inhibitor present in the sample is blocked and the protease inhibitor is removed from the inhibited form of cathepsin B, and the activity of the active cathepsin B present in the sample is subsequently determined.</p> |