QUANTIFICATION METHOD FOR TOTAL AMOUNT OF MICROALGAL LIPIDS BY NEAR-INFRARED RAMAN SPECTROMETRY

摘要

A quantification method for a total amount of microalgal lipids by near-infrared Raman spectrometry is disclosed, which includes the following steps: providing a microalgae sample and a substrate of which a surface is covered by a metal layer; applying the microalgae sample on the metal layer of the substrate; supplying a laser light in a wavelength of near-infrared light by which the microalgae sample is excited; recording Raman signals of the microalgae sample to form a Raman spectrum; and converting intensity of lipid signals in the Raman spectrum into a total amount of microalgal lipids in the microalgae sample according to a calibration curve.