PRIMER SETS FOR DETECTING BEGOMOVIRUS BETA-SATELLITE DNA AND METHOD FOR DETECTING BEGOMOVIRUS BETA-SATELLITE DNA USING THE SAME

摘要

PURPOSE: A primer set for detecting begomovirus beta satellite DNA and a method for detecting begomovirus beta satellite DNA is provided to have different DNA host plants, capable of detecting a respective primer set, thereby specifically detecting diagnosis of some begomovirus. CONSTITUTION: A primer set for detecting begomovirus beta satellite DNA is selected from a primer set of sequence number 1 and sequence number 2, a primer set of sequence number 3 and sequence number 4, a primer set of sequence number 5 and sequence number 6, and a primer set of sequence number 7 and sequence number 8. The primer set of sequence number 1 and sequence number 2 is for generally detecting begomovirus beta satellite DNA. The primer set of sequence number 3 and sequence number 4 is for detecting HYVMV(Honeysuckle yellow vein mosaic virus). The primer set of sequence number 5 and sequence number 6 is for detecting HYVV(Honeysuckle yellow vein virus). A method for detecting begomovirus beta satellite DNA comprises a step of separating total DNA from a plant sample; a step of amplifying a target sequence by using a primer set; and a step of detecting the amplified product.