Process for the isolation and stabilization of low molecular weight aminoglycans from waste egg shells

摘要

Processes for the isolation of low-molecular weight aminoglycan compound of formula I from a hitherto unexploited natural source of waste egg shells which is made up of alternating glucuronic acid and N-acetyl glucosamine units comprising the steps of: (a) pre-preparation of the waste egg-shells for extraction of the embryonic low molecular weight aminoglycan compound of formula I using a polar organic solvent in water; (b) extraction of the low molecular weight aminoglycan compound of formula I as its soluble salt using an aqueous polar salt solution; (c) isolation of a purified low molecular weight aminoglycan compound of formula I by gel formation out of the aqueous salt mixture by using a polar organic solvent followed by filtration or centrifuging; (d) stabilization of the isolated aminoglycan extract by sequential introduction of organic oils into a semi-dried gel to form ordered sheets of aminoglycan compound of formula I with no visible cross-linking of the carbohydrate polymer.