发明名称 |
Nucleotide sequences with modified initiation codon for the translation ofaav-rep78 useful for production of aav in insect cells and cells comprising same |
摘要 |
The present invention relates nucleic acid constructs for the production of recombinant parvoviral (e.g. adeno-associated viral) vectors in insect cells, to insect cells comprising such constructs and to methods wherein the cells are used to produce recombinant parvoviral virions. The insect cells preferably comprise a first nucleotide sequence encoding the parvoviral rep proteins whereby the initiation codon for translation of the parvoviral Rep78 protein is a suboptimal initiation codon that effects partial exon skipping upon expression in insect cells. The insect cell further comprises a second nucleotide sequence comprising at least one parvoviral (AAV) inverted terminal repeat (ITR) nucleotide sequence and a third nucleotide sequence comprising a sequences coding for the parvoviral capsid proteins. |
申请公布号 |
IL196091(A) |
申请公布日期 |
2013.04.30 |
申请号 |
IL20080196091 |
申请日期 |
2008.12.21 |
申请人 |
WILHELMUS THEODORUS JOHANNES MARIA CHRISTIAAN HERMENS;SASKIA JACOBA PETRONELLA HAAST;DENNIS JOHAN BIESMANS;ANDREW CHRISTIAN BAKKER;UNIQURE IP B.V.;UNIQURE BIOPHARMA B.V. |
发明人 |
WILHELMUS THEODORUS JOHANNES MARIA CHRISTIAAN HERMENS;SASKIA JACOBA PETRONELLA HAAST;DENNIS JOHAN BIESMANS;ANDREW CHRISTIAN BAKKER |
分类号 |
C12N |
主分类号 |
C12N |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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