发明名称 |
METHOD FOR CONSTRUCTING RECOMBINANT BACTERIUM FOR PRODUCING NON-NATIVE PROTEIN, AND UTILIZATION OF SAME |
摘要 |
The present invention provides a novel method of producing a recombinant bacterium for production of a non-natural protein, including: (1) expressing tRNA in a bacterium, which tRNA recognizes UAG codon; (2) expressing an aminoacyl-tRNA synthetase in the bacterium, which aminoacyl-tRNA synthetase acylates the tRNA with a non-natural amino acid or an ±-hydroxy acid; (3) (i) introducing a DNA construct into the bacterium, which DNA construct is for expressing, in the absence of a release factor for terminating translation at UAG codon, a function of at least one gene selected from the group consisting of genes each of which loses its function when a gene that codes for the release factor is defective and/or introducing an alteration into said at least one gene in a chromosome of the bacterium, which alteration is for expressing the function of said at least one gene in the absence of the release factor; and (4) causing the gene that codes for the release factor in the bacterium to be defective. |
申请公布号 |
EP2584037(A1) |
申请公布日期 |
2013.04.24 |
申请号 |
EP20110795797 |
申请日期 |
2011.06.16 |
申请人 |
RIKEN |
发明人 |
YOKOYAMA, SHIGEYUKI;MUKAI, TAKAHITO;SAKAMOTO, KENSAKU;MATSUMOTO, AKIKO |
分类号 |
C12N15/09;C12N1/21;C12N9/10;C12P21/02 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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